sábado, 13 de outubro de 2012

Nigéria: Researcher identifies local plants with antifungal properties


A Nigerian researcher has identified no fewer than 520 local plants that could be used to treat fungal skin infections, control water hyacinth, biodegrade crude oil spill, prevent dental decay, and control uterine contraction and bleeding during child birth.

A PROFESSOR of Botany at the University of Lagos, Adedotun Adeyinka Adekunle, has documented 520 plant species with proven antifungal properties.

Adekunle in his Inaugural lecture, titled “Fungi: Friends or Foes to Mankind,” delivered last week at the University’s main auditorium, said: “Several studies were carried out on the purification of the antifungal principle from Nigerian plants. The active antifungal ingredient was tested on human fungal pathogens (dermatophytes) as well as on plant pathogens. It is always necessary to develop new natural drugs because of resistance to existing drugs by microorganisms like fungi.

“A survey of medicinal plants used in the treatment of skin rashes, eczema, athlete foot and ring worm in the south western, Nigeria was carried out. A medicinal plant list of 520 plant species with antifungal properties was documented.”

Adekunle who specializes in mycology (the study of fungi) said phytochemical properties of some of the plant extracts were investigated and were found to contain flavonoid, saponins, terpenes and tannins with fungicidal properties on dermatophytes such as Epidermophyton floccosom, Mucor mucedo (opportunistic), Microsporium audonii, and Trichophyton verrucosum.

Adekunle and his team of researchers also investigated the antifungal activity of four Nigerian chewing sticks from the stem or root of Angeissus schimperi (ayin in Yoruba), Distemonathus benthmianus (ayan in Yoruba), Vernonia amagdalina (orin ewuro in Yoruba, bitter leaf) and Zanthoxylum xanthoxyloides (orin ata in Yoruba).

“This study was set up to further scientifically support the use of chewing sticks in dental care by the Nigerian natives. It was found that the extracts of these chewing sticks were potent against fungi used,” he said.

The mycologist and his team also carried out another study on the time of leaf harvest (in a 24 hour rhythm) and antifungal activity of Acalypha wilkesiana (lara pupa in Yoruba) leaf extracts. Acalypha wilkesiana is used to treat skin rashes in babies (ela in Yoruba).

He explained: “The Nigerian traditional medicine practitioner believe that plants sleep at night between 7pm to 5am for their medicinal purpose. However, our study discovered that the best period of harvest of the Acalypha wilkesiana leaf was 3am. The leaves harvested at 3am had the highest concentration of corilagin and geranin, which are the antifungal active ingredients hence had the highest zone of inhibition on the fungi.

“There was a definite change in the antifungal activity of extracts of Acalypha wilkesiana leaves harvested at different times of the day (3am, 6am, 9am, 12noon, 3pm, 6pm, 9pm and 12 midnight). The least activity was recorded from leaves harvested at 12noon. Our report did not agree with the claims of the Nigerian natives to harvest plants for medicinal purpose only during the day light in the case of Acalypha wilkesiana leaf.”

Adekunle and his team also studied the efficacy of preserving plant seeds against fungi using wood ash of some tropical trees in Nigeria. The wood ash of nine Nigerian trees: Khaya grandifolia; Nauclea diderrichi; Piptadeniastrum africanum; Mangifera indica; Mansonia altissima; Triplochiton scleroxylon; Ceiba pentandra; Terminalia superba; and Terminalia ivoriensis were used to preserve beans, maize and melon seeds.

“The seeds stored with ashes of Nauclea diderrichi and Piptadeniastrum africanum were the most effective stopping fungal growth and eliminating weevils. The wood ashes used compared favourably with the orthodox fungicides benlate. The wood ash of these plants contained flavonoids and tannins which stop fungal infection and preserve the seeds.”

Adekunle with his colleagues from the department of biochemistry have isolated a pathogenic fungus called Myrothecium roridum that is able to destroy the water hyacinth, ‘eat up’ the whole plant, within five days post inoculation on the leaves. “The fungus can thus control the water hyacinth. The fungal action is systemic and host specific. On further investigation we isolated and purified a phytotopxin and mycoherbicide from Myrothecium roridum. With this discovery in 2006, we won the Faculty Best Researchers Award at the University of Lagos research fair. We have sent our findings and proposal to the Ministry of environment, Lagos state for further discussions on the biocontrol of water hyacinth in Lagos waters using this fungus,” he explained.

Adekunle and his team have also investigated the potential of alkaloids from Panicum maximum floret infected with the fungus Tilletia ayresii in the control of uterine contraction using bioassay guided fractionation technique.

They wrote: “Our aim of conducting this research was to discover cheaper and easily accessible drugs of Nigerian origin for controlling uterine contraction, which led to expulsion of placenta and subsequent control of bleeding after childbirth. The only source of the uterine contraction drug, ergometrine or ergot alkaloids before our study was from ergot or rye, a temperate plant that cannot grow in the tropics, Nigeria inclusive. Hence, it is imported with scarce foreign exchange.

“Ergot of rye is an infected rye floret by the fungus Claviceps purpurea. Claviceps purpurea infects the ovary of its host, rye, just as Tilletia ayresii infects the ovary of its own host, Panicum maximum (Guinea grass), a tropical plant found in Nigeria.

“We discovered an alkaloid as the active ingredient responsible for the uterine contraction in rats, from infected Panicum maximum floret purified extract. This discovery has been registered as a patent with the National Office of Technology Acquisition Promotion Agency (NOTAP).”

The botanist said his team has conducted a research to source fungi capable of biodegrading the petroleum oil from some Nigerian oilseeds such as soybean, maize, melon, Detarium senegalense, Treculia africana, and Irvingia gabonensis (Ogbonno in Yoruba). “We discovered that the pathogenic fungi isolated from the oil within 40 days. The oilseeds contain triacylglyceride, which is the vegetable oil hydrocarbon and it is biodegradable by the pathogenic fungi. This group of fungi was also able to biodegrade the hydrocarbon in the petroleum oil,” Adekunle said.

Adekunle in his inaugural lecture made several recommendations on how to improve scientific research in Nigeria. The professor of botany called for the establishment of a fungal collection centre to help in the confirmation of fungal identification. “Cultures are usually sent to America or United Kingdom. It used to be free, but now fungal confirmation attracts heavy fees in foreign currency.”

Adekunle want the government to introduce encouragement of research through financial reward for their work as practiced in South Africa. He said there is urgent need to establish a functional central laboratory for research in the University; promote and establish functional scholarship awards for post-graduate studies in all disciplines; and build accommodation for both members of staff and students; devise newer ways of providing sustainable electricity supply to the University.

Adekunle concluded: “These are my contributions in mycology, making fungi friends of man voluntarily or compulsorily scientifically. Fungi are indeed friends to humans.”

Data: 10.11.2012
Texto: Chukwuma Muanya

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