Shi, Y.; Rupa, P.; Jiang, B.; Mine, Y. Hydrolysate from Eggshell Membrane Ameliorates Intestinal Inflammation in Mice. Int. J. Mol. Sci. 2014, 15, 22728-22742.
Abstract
Inflammatory bowel diseases (IBD) comprises of ulcerative colitis (UC) and Cohn’s disease (CD) as two main idiopathic pathologies resulting in immunologically mediated chronic inflammatory conditions. Several bioactive peptides and hydro lysates from natural sources have now been tested in animal models of human diseases for potential anti-inflammatory effects. Eggshell membrane (ESM) is a well-known natural bioactive material. In this study, we aim to study the anti-inflammatory activity of ESM hydro lysate (AL-PS) in vitro and in vivo. In vitro, AL-PS was shown to inhibit pro-inflammatory cytokine IL-8 secretion. In vivo treatment with AL-PS was shown to reduce dextran sodium sulphate (DSS)-induced weight loss, clinical signs of colitis and secretion of interleukin (IL)-6 (p < 0.05). In addition, treatment with AL-PS also attenuated the severity of intestinal inflammation via down-regulation of IL-10 an anti-inflammatory cytokine. This validates potential benefits of AL-PS as a novel preventative target molecule for treatment of IBD.
Conclusions
In conclusion, this study validates possible mechanism of anti-inflammatory activity of AL-PS derived from ESM in vivo. Administration of AL-PS ameliorated DSS-induced colitis signs and severity of inflammation in mice via down-regulation of pro-inflammatory cytokine expression and boosting T cells apoptosis to restore immune homeostasis in gut, suggesting that AL-PS may be a promising preventative target for curing intestinal inflammation, such as IBD. Though there is limitation in current study, it has important implication for providing with a solution to use eggshell membrane as high value-added material to produce preventative products with anti-inflammatory activity.
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